POLYMERASE CHAIN REACTION

Ortiqova Tursunoy Avaz kizi; Raxmankulova Sayyora Raimkul kizi; Mukhamedova Sevara Nigmatulla kizi

doi:10.5281/zenodo.11094531

Ortiqova Tursunoy Avaz kizi, Raxmankulova Sayyora Raimkul kizi, Mukhamedova Sevara Nigmatulla kizi

Abstract. The method is based on the detection of even small concentrations of the desired diagnostic element. To determine the initially extremely small concentrations of RNA or DNA that need to be determined during the main stage of the study, a method is used to artificially increase the amount of RNA or DNA.

Keywords: PCR, RNA, DNA, replication, polymerase, Recombinase Polymerase Amplification, Transcription, genetic fingerprints, asymmetric PCR, Reverse Transcription PCR, Inverse PCR.

References:

Kleppe, K. et al. (1971): Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA’s as catalyzed by DNA polymerases. In: J. Mol. Biol. Bd. 56, pp. 341–361. PMID 4927950 2. 2.Bartlett, J. M. S.; Stirling, D. A Short History of the Polymerase Chain Reaction // PCR Protocols (English). — 2nd. - 2003. - Vol. 226. - P. 3-6. - (Methods in Molecular Biology). — ISBN 1-59259-384-4. - doi:10.1385/1-59259-384-4:3. 3. Mullis, Kary B. et al. “Process for amplifying, detecting, and/or-cloning nucleic acid sequences” U.S. Patent 4 683 195 4. Saiki R.K., Scharf S., Faloona F., Mullis K.B., Horn G.T., Erlich H.A., Arnheim N. Science 1985 Dec 20; 230 (4732): 1350-4; Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. 5. 5.[Nobel Laureates in Chemistry, 1993 (English). Access date: August 29, 2007. Archived October 26, 2012. Nobel laureates in chemistry, 1993 (English)] 6. R. K. Saiki, D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis, H. A. Erlich. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase Archived December 19, 2008 on the Wayback Machine. in: Science. 239.1988, 487-491. ISSN 0036-8075 PMID 2448875 7. Kaledin A. S., Slyusarenko A. G., Gorodetsky S. I. // Biochemistry. — 1980. — T. 45. — P. 644—651