EFFECT OF NITROGEN COMPONENTS ON GROWTH AND PIGMENT FORMATION OF MICROALGAE CHLORELLA VULGARIS 4 AND SCENEDESMUS QUADRICAUDA 32

Kayumova Iroda Komolovna; Kadirova Gulchekhra Khakimovna; Shonakhunov Tulkin Erkinovich

doi:10.5281/zenodo.11068321

EFFECT OF NITROGEN COMPONENTS ON GROWTH AND PIGMENT FORMATION OF MICROALGAE CHLORELLA VULGARIS 4 AND SCENEDESMUS QUADRICAUDA 32

25.04.2024 International Scientific Journal "Science and Innovation". Series D. Volume 3 Issue 4

Kayumova Iroda Komolovna, Kadirova Gulchekhra Khakimovna, Shonakhunov Tulkin Erkinovich

Abstract. The utilization of microalgae in wastewater treatment leads to wastewater bioremediation, which can be used to produce algal by-products. However, to evaluate the growth of microalgae, many factors have to be considered, mainly the cultivation conditions. In this context, the components of the medium affect the direct growth of microalgae and consequently the biomass yield. In this work, the cultivation conditions of native microalgae strains Chlorella vulgaris 4 and Scenedesmus quadricauda 32 were optimized to obtain the highest biomass and pigments. It was shown that the formation of dry biomass of C.vulgaris 4 on the standard medium "Tamiya" is 358 mg/l, and on the medium with increased content of nitrogen components in 2 times - 424 mg/l of medium. Consequently, the increase in the nitrogen component of the medium increases the biomass formation of C. vulgaris 4 by 18.4%. Under the same conditions, the formation of dry biomass by the S. quadricauda 32 culture increases by 24.3%. When growing C. vulgaris 4 and S. quadricauda 32 on the medium "Chu-13" with increased content of nitrogen components in 2 times the formation of dry biomass exceeds the biomass formed when growing on standard medium by 16% and 14.3%, respectively. It was revealed that the content of chlorophyll and carotenoids in C. vulgaris 4 and S. quadricauda 32 increases in "Tamiya" and "Chu-13" media with increased content of nitrogen components 2 times. In particular, the content of chlorophyll a, chlorophyll b and carotene in C. vulgaris 4 grown on "Tamiya" medium increased by 12.5%, 18.9% and 20%, respectively. Thus, the analysis of the research results showed that the quality of nutrient medium influences the formation of biomass and pigments used in the cultivation of microalgae.

Keywords: microalgae, biomass, nitrogen components, Chlorella vulgaris, Scenedesmus quadricauda, chlorophyll a, chlorophyll b, carotene

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