Abstract. The article describes in detail the epizootic situation regarding ascospherosis in the Fergana Valley. The number of bee colonies affected by ascospherosis is 5.9 – 35.0% of the total number of those studied. Clinical manifestations of the disease in the southern regions of the Fergana Valley are observed in early March, and in the Central regions - in April. In the Southern regions of the Fergana Valley, the incidence of bees with ascospherosis is mainly manifested by weak, in the Central - medium and severe degrees of infection of the brood.