Abstract. The study focused on the capacity of indigenous strains of lactic acid bacteria to synthesize branched-chain amino acids, a pivotal factor in determining the formulation of a biopreparation designed for the prevention and treatment of obesity. Notably, within the array of strains examined, L. casei K7/3 demonstrated a remarkable twofold increase in branched-chain amino acid production when cultivated in MRS-bouillon medium. Specifically, there was a significant elevation in leucine content, a deficiency or absence of which in the human body can precipitate a decline in body weight, impede development and growth, and lead to metabolic disturbances.
References:
1. Glagoleva L.E, Rуаskinа L.O., Rodionov A.A., Pastukhov N.A. Prospects of innovative products on the basis of a healthy diet supplement "Vitazar". Vestnik VGUIT. [Proceedings of Voronezh State University engineering technology] 2016, no. 1 (67), pp. 122–127. (inRussian)
2. Ogay D.K., Miralimova Sh.M., Kutlieva G.J., Elova N.A. No. IAP2015 0326 Biologically active supplement (BAA) for the prevention and therapy of gastrointestinal ulcer diseases.
3. ElovaN.A.,Kutliyeva G.J., ZakiryaevaS.I., BekmukhamedovaN.K. Optimization of cultivation conditions for increasing the production of exopolysaccharides of the Lactobacillus plantarum EB-2 strain // Natural Volatiles & Essential Oils. 2021, 8(5): 8689-8697.
4. AzizovD. Z., RakhmanberdievaR. K., ElovaN.A. Рolysaccharides from roots of Fеrulatenuisecta and their antimicrobial activity // Chemistry of Natural Compounds Chemistry of Natural Compounds, DOI 10.1007/s10600-022-03747-1 Vol. 58, No. 4, July, 2022. Pp. 589-592.
5. Grahame Hardie, D. AMP-activated protein kinase: A key regulator of energy balance with many roles in human disease. J. Intern. Med. 2014, 276, 543–559.
6. Bai J., Greene E., Li W., Kidd M.T., Dridi S. Branched-chain amino acids modulate the expression of hepatic fatty acid metabolism-related genes in female broiler chickens. Mol. Nutr. FoodRes. 2015, 59, 1171–1181.
7. Medras Z.J.H., El-Sayed N.M., Zaitone S.A., Toraih E.A., Samie M.M., Moustafa Y.M. Glutamine up-regulates pancreatic sodium-dependent neutral aminoacid transporter-2 and mitigates islets apoptosis in diabetic rats. Pharmacol. Rep. 2017, 70, 233–242.
8. Ma X., Han M., Li D., Hu S., Gilbreath K.R., Bazer F.W., Wu G. L-Arginine promotes protein synthesis and cell growth in brown adipocyte precursor cells via the mTOR signal pathway. Amino Acids 2017, 49, 957–964.
9. Steven A., Cohen Daviel J. Amino acid Analysis Utilizing Phenylisothiocyanata derivatives// Analytical Biochemistry. — 1988. — №1b. — P.1-16.