CELLULOLYTIC AND ANTIMICROBIAL PROPERTIES OF SOME BACTERIA ISOLATED FROM DOMESTIC ANIMALS

18.12.2023 International Scientific Journal "Science and Innovation". Series D. Volume 2 Issue 12

Kutlieva G.J., Turaeva B.I., Kamolova H.F, Haydarov B.H.

Abstract. In recent years, there has been an interest among researchers in the search for new microorganisms with probiotic properties. Among them, many studies in this regard are devoted to Bacillus subtilis, which is widespread in nature and is not pathogenic for animals and humans. Some results of a study of the biological properties and antagonistic activity of Bacillus subtilis are presented, which was carried out with the aim of developing methodological approaches to identifying strains with antagonistic activity against certain types of opportunistic microorganisms and their further use as probiotic preparations. According to cultural, morphological and biochemical characteristics, the studied bacterial strains corresponded to the species characteristics of Bacillus subtilis and were confirmed by genetic identification using the 16 S rRNA method. As a result of the experiments, the antagonistic activity of Bacillus subtilis strains against Staphylococcus aureus, Candida albicans, and E. coli was established. The growth inhibition zone of these crops ranged from 15 to 25 mm. The studied Bacillus subtilis strains can be proposed for use as probiotics

Keywords: microorganisms, cellulose, probiotics, antimicrobial activity, opportunistic bacteria, carboxymethylcellulose, cellobiose