Abstract. The work studied the composition of flavonoids of the great plantain (Plantago major L.), growing in the Republic of Uzbekistan. The initial raw materials were collected in the Chust district of the Namangan region in mid-September. From this raw material, 104.4 g (5.22%) of polyphenolic compounds were obtained by extraction with organic solvents. Next, by column chromatography, 3 fractions were obtained - diethyl ether (1-fraction), aqueous (2-fraction) and acetone (3-fraction). Substances from the fractions were separated using paper and high-performance liquid chromatography. The presence of 5 flavonoids in P. major L. was shown: rutin, luteolin, isorhamnetin, quercetin-3-O-β-D-galactopyranoside and 3,5,7,3'4'-pentaoxyflavone (quercetin). It is concluded that this type of plant is promising in terms of developing new medicines for the treatment of various pathologies.