DETERMINATION OF BIOLOGICAL ACTIVITY OF CYNARA SCOLYMUS L. ROOT EXTRACT AGAINST HARMFUL MICROORGANISMS IN THE TERRITORY OF UZBEKISTAN

Kh.Kh. Dolimov; I.J. Jalolov; M.Y.Imomova; M.R.Mamadaliyev

doi:10.5281/zenodo.8029301

DETERMINATION OF BIOLOGICAL ACTIVITY OF CYNARA SCOLYMUS L. ROOT EXTRACT AGAINST HARMFUL MICROORGANISMS IN THE TERRITORY OF UZBEKISTAN

12.06.2023 International Scientific Journal "Science and Innovation". Series D. Volume 2 Issue 6

Kh.Kh. Dolimov, I.J. Jalolov, M.Y.Imomova, M.R.Mamadaliyev

Abstract. The biologic activity of extracts obtained from the leaves of Cynara scolymus L., belonging to the Asteraceae family, using ethyl alcohol and low polar solvents, was investigated against Gram-positive bacteria (Staphylococcus aureus ATCC 25923 and Bacillus subtilis RKMUz-5), Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27879 and Escherichia coli RKMUz-221), and yeast (Candida albicans RKMUz-247). As a result of the research, it was found that extracts obtained using ethyl alcohol exhibited high activity against Gram-positive bacteria

Keywords: Cynara scolymus L., Asteraceae, biologic properties, secondary metabolites, Gram-positive, Gram-negative, bacteria, extract

References:

1. Antibacterial, antifungal and cytotoxic activites of eight asteraceae and two rubiaceae plants from Columbian biodiversity: Jaim N, Diana M Narvaez, Oscar M.Mosquera. 2007. 2. Paula, J. C. N. V. F., David, J. M., David, J. P. Occurrence, biological activity and 13C NMR data of amides from Piper (Piperaceae). Quim. Nova, 2012, 35, p. 2288-2311 3. Яковлев, С.А. Инфекционные заболевания как глобальная проблема современности/ С.А. Яковлев// Территория науки. - 2017. С. 98-113. 4. Al-Taweel, A.M., Perveen, S., El-Shafae, Fawzy, G.A., Malik, A., Afza., Iqbal, L., Latif, M. Bioactive phenolic amides from Celtis Africana. Molecules 2012, 17, 2675 – 2682. 5. Масленников П.В., Чупахина Г.Н., Скрыпник Л.Н. Содержание фенольных соединений влекарственных растениях ботанического сада // Известия Российской академии наук. Сериябиологическая. 2013. С. 47-62. 6. Atanasov, A. G., Waltenberger, B., Pferschy-Wenzig, E. M., Linder, T., Wawrosch, C., Uhrin, P., Temml., Wang, L., Scwaiger, S., Heiss, E.H., Rollinger, J.M., Schuster, D., Breuss, J. M., Bochkov, V., Mihovolovic, M. D., Kopp, B., Bauer, R., Dirsch, V. M., Stuppner, H. Discovery and resupply of pharcologically active plant-derived natural products: a review. Biotechnology advances 2015, 1582 – 1614. 7. Ávila, H.P., De Fátima Albino Smânia, E., Monache, F.D, Júnior, A.S. Structure-activity relationship of antibacterial chalcones. Bioorg. Med. Chem. 2008, 16, 9790 – 9794. 8. Balunas, M. J., Kinghorn, A. D., Drug discovery from medicinal plants. Life Sci. 2005, 78, 431 – 441. 9. Brielman, H. L., Setzer, W. N., Kaufman, P. B., Kirakosyan, A., Cseke, L. J. Phytochemicals: The chemical components of plants, in Natural products from plants 2nd.Taylor & Francis Group, Boca Raton. 2006, 22 – 25. 10. Büchi, G., Crombie, L., Godin, P. J., Kaltenbronn, J. S., Siddalingaiah, K. S., Whiting, D. A. The absolute configuration of rotenone. J. Chem. Soc. 1961, 2843 – 2840. 11. Chin, Y. W., Mdee, L. K., Mbwambo, Z. H., Mi, Q., Chai, H. H., Cragg, G. M., Swanson, S.M., Kinghorn, A. D. Prenylated flavonoids from the root bark of Berchemia discolor, a Tanzanian medicinal plant. J. Nat. Prod. 2006, 69, 1649 – 1652. 12. Christenhusz, M. J. M., Byng, J. W. The number of known plants species in the world and its annual increases. Phytotaxa. 2016, 261, 201 – 217. 13. CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standart – Ninth Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standarts Institute; 2012.