CLONING A FOREIGN GENE IN THE YEAST PICHIA PASTORIS

O.Ashirov; T.Sadullaev; A.Yarilkaganova; A.Normatov S.Sasmakov Sh.Azimova

doi:10.5281/zenodo.7441236

O.Ashirov, T.Sadullaev, A.Yarilkaganova, A.Normatov S.Sasmakov Sh.Azimova

Abstract. The S gene encoding the hepatitis B surface antigen was cloned into the yeast Pichia pastoris using the pPIC9 transfer vector. The recombinant plasmid pPIC9-S was treated with the restriction enzyme SacI and inserted into the yeast Pichia pastoris via electroporation. Yeast clones were selected by growing in a medium without histidine. As a result, Mut+ phenotypes of Pichia pastoris containing the S gene were obtained.

Keywords: Pichia pastoris, recombinant DNA, S gene, transfer vector, electroporation, restriction, transformation.

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